【文獻(xiàn)標(biāo)題】Replacing fish oil with palm oil: Effects on mRNA expression of fatty acid transport genes and signalling factors related to lipid metabolism in Nile tilapia (Oreochromis niloticus)

【作者】Christian Larbi Ayisi,Jin-Liang Zhao,Xue-Ming Hua,et.al

【作者單位】上海海洋大學(xué)（Shanghai Ocean University）

【文獻(xiàn)中引用產(chǎn)品】

魚(yú)過(guò)氧化物酶體增殖物激活受體α(PPAR-α)ELISA試劑盒

魚(yú)過(guò)氧化物酶體增殖物激活受體β(PPARβ)ELISA試劑盒

魚(yú)脂蛋白酯酶（LPL）ELISA試劑盒

魚(yú)脂肪酸結(jié)合蛋白3（FABP3）ELISA試劑盒

魚(yú)金屬忍耐蛋白(MTP)ELISA試劑盒

魚(yú)可溶性肝X受體(LXR)ELISA試劑盒

魚(yú)脂肪酸延長(zhǎng)酶5(ELOVL5)ELISA試劑盒

魚(yú)葡萄糖6磷酸脫氫酶(G6PD)ELISA試劑盒

魚(yú)蘋(píng)果酸酶（ME）ELISA試劑盒

【關(guān)鍵詞】ELOVL 5, fatty acid metabolism, fatty acid transport, glucose 6-phosphate dehydrogenase, 

lipoprotein lipase, palm oil

【DOI】doi.org/10.1111/anu.12821

【出版期刊】《Aquaculture Nutrition》

【產(chǎn)品原文引用】

Enzyme activities

Enzyme-linked immunosorbent assay (ELISA) was used to measure the enzyme activities of PPAR-α, PPAR-β, LPL, FABP-3, MTP, LXR, ELOVL 5, 6GPD, G6PD and ME with a commercial kit from Shanghai Ding Biological Technology Company Limited (Shanghai, China). A ground glass homogenizer was used to homogenize liver (0.5-1.0 g) on ice. The homogenates were centrifuged (110 000 g, 20 min at 4°C), and the clear phase between the top layer and the pellets was used for the analysis. At 450 nm, an ELISA microplate reader was used to measure the optical density (OD) of samples in a 96-well plate. According to the manufacturer’s instructions, a standard curve was generated and the standard diversity calculated using Excel 2003. Enzyme activity units (IU), defined as moles of substrate, were converted into product per minute at assay temperature.